Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - other (Yeast membrane preparation)
Loading amount
5 µg
Specification
Yeast membrane preparation
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/7000
Incubation time
2 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TBS+tween
Secondary antibody
Name
Dilution
1/45000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 70, 50, 35 kDa
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提交于 Aug 07 2009