重组Anti-Plectin抗体[E398P] (ab32528)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E398P] to Plectin
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Plectin抗体[E398P]
参阅全部 Plectin 一抗 -
描述
兔单克隆抗体[E398P] to Plectin -
宿主
Rabbit -
特异性
This antibody recognises plectin, a 500-kDa intermediate filament binding protein.
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经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat whole cell lysate (ab7899), human skeletal muscle WB: U-2 OS cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E398P -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32528于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/5000. Predicted molecular weight: 532 kDa.
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说明 |
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IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/5000. Predicted molecular weight: 532 kDa. |
靶标
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功能
Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity. -
组织特异性
Widely expressed with highest levels in muscle, heart, placenta and spinal cord. -
疾病相关
Epidermolysis bullosa simplex with pyloric atresia
Epidermolysis bullosa simplex, with muscular dystrophy
Epidermolysis bullosa simplex, Ogna type
Limb-girdle muscular dystrophy 2Q -
序列相似性
Belongs to the plakin or cytolinker family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 33 plectin repeats.
Contains 4 spectrin repeats. -
结构域
The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4. -
翻译后修饰
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis. -
细胞定位
Cytoplasm > cytoskeleton. Cell junction > hemidesmosome. - Information by UniProt
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数据库链接
- Entrez Gene: 5339 Human
- Entrez Gene: 18810 Mouse
- Entrez Gene: 64204 Rat
- Omim: 601282 Human
- SwissProt: Q15149 Human
- SwissProt: Q9QXS1 Mouse
- SwissProt: P30427 Rat
- Unigene: 434248 Human
see all -
别名
- EBS1 antibody
- EBSO antibody
- HD1 antibody
see all
图片
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Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Plectin antibody [E398P] (ab32528) at 1/5000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : PLEC knockout U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 532 kDaab32528 was shown to react with PLEC in wild-type U-2 OS cells in Western blot with loss of signal observed in a PLEC knockout cell line. Wild-type U-2 OS and PLEC knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab32528 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab32528 被引用在 33 文献中.
- Mouron S et al. Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A. Nat Commun 13:7529 (2022). PubMed: 36477027
- Xu R et al. Plectin Downregulation Inhibits Migration and Suppresses Epithelial Mesenchymal Transformation of Hepatocellular Carcinoma Cells via ERK1/2 Signaling. Int J Mol Sci 24:N/A (2022). PubMed: 36613521
- Buckup M et al. Plectin is a regulator of prostate cancer growth and metastasis. Oncogene 40:663-676 (2021). PubMed: 33219316
- Baek A et al. KRT8 (keratin 8) attenuates necrotic cell death by facilitating mitochondrial fission-mediated mitophagy through interaction with PLEC (plectin). Autophagy 17:3939-3956 (2021). PubMed: 33783309
- Evtushenko NA et al. hTERT-Driven Immortalization of RDEB Fibroblast and Keratinocyte Cell Lines Followed by Cre-Mediated Transgene Elimination. Int J Mol Sci 22:N/A (2021). PubMed: 33916959