Application
Western blot
Sample
Human Purified protein (Blood)
Loading amount
3 µg
Specification
Blood
Gel Running Conditions
Reduced Denaturing (4-20% Tris HCl gels)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 0.05% TBST
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
No secondary antibody was used, however the membrane was incubated with streptavidin linked-HRP diluted 1/500 in TBST for 1 hour at room temperature.
The band at ~60 kDa represents the plasmin heavy chain.
The band at ~60 kDa represents the plasmin heavy chain.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Rachel Barker
Verified customer
提交于 Aug 10 2009