Application
Western blot
Sample
Pig Purified protein (porcine plasma, porcine articular capsule extract)
Gel Running Conditions
Non-reduced Denaturing (10% PAGE)
Loading amount
0.2 µg
Specification
porcine plasma, porcine articular capsule extract
Blocking step
Tween 20 as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 0.05%Tween 20/0.5% milk/0.01% NaN3/PBS
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: DAKO D487 AP-conj. goat anti-rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
NBT-BCIP
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: double bands~85 + fr kDa
Positive control
Abcam plasma kallikrein (KLKB1, ab77870)
Additional data
Additional Notes
PVDF membrane, semi-dry blot
Isolation of plasma kallikrein (KLKB1) from human plasma, porcine plasma and porcine articular capsule extract using pretreatment and affinity chromatography essentially according to Nagase & Barrett, Biochem. J. 193:187-192 (1981)
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提交于 Feb 10 2016