Application
Western blot
Sample
Human Recombinant protein (Human recombinant hPL)
Loading amount
0.1 µg
Specification
Human recombinant hPL
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG whole antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~25 kDa Non-specific: weak ~10, 14, 45 kDa
Positive control
None
Negative control
None
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Stoil Dmitrov
Verified customer
提交于 Apr 11 2007