重组Anti-Placental alkaline phosphatase (PLAP)抗体[SP15] (ab16695)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Placental alkaline phosphatase (PLAP)抗体[SP15]
参阅全部 Placental alkaline phosphatase (PLAP) 一抗 -
描述
兔单克隆抗体[SP15] to Placental alkaline phosphatase (PLAP) -
宿主
Rabbit -
特异性
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
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经测试应用
适用于: Flow Cyt (Intra), IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is considered to be commercially sensitive.
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表位
Not determined -
阳性对照
- IHC-P: Seminoma and placenta tissues. Flow Cyt (intra): HeLa cells
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常规说明
This product was switched from a hybridoma to recombinant production method on 7th March 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). -
克隆
单克隆 -
克隆编号
SP15 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/200.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
说明 |
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Flow Cyt (Intra)
1/200. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
靶标
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序列相似性
Belongs to the alkaline phosphatase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 250 Human
- Entrez Gene: 18786 Mouse
- Entrez Gene: 100359675 Rat
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
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别名
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
see all
图片
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Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
This image was generated from the hybridoma version.
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Human seminoma stained with ab16695.
This image was generated from the hybridoma version.
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Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab137879 at 1/50 dilution.
This image was generated from the hybridoma version.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab16695 被引用在 11 文献中.
- Zhou Y et al. The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways. Stem Cell Res Ther 12:64 (2021). PubMed: 33461605
- Guo XY et al. Calnexin mediates the maturation of GPI-anchors through ER retention. J Biol Chem 295:16393-16410 (2020). PubMed: 32967966
- Cardot-Ruffino V et al. Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain. Genesis 58:e23359 (2020). PubMed: 32191380
- Ashley J et al. Transsynaptic interactions between IgSF proteins DIP-a and Dpr10 are required for motor neuron targeting specificity. Elife 8:N/A (2019). PubMed: 30714906
- Bracey DN et al. Investigating the Osteoinductive Potential of a Decellularized Xenograft Bone Substitute. Cells Tissues Organs 207:97-113 (2019). PubMed: 31655811