Anti-Phospholipase D1抗体[EP1506Y] (ab68150)
Key features and details
- Rabbit monoclonal [EP1506Y] to Phospholipase D1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Phospholipase D1抗体[EP1506Y]
参阅全部 Phospholipase D1 一抗 -
描述
兔单克隆抗体[EP1506Y] to Phospholipase D1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, NIH/3T3 and C6 whole cell lysate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1506Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68150于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
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说明 |
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WB
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
靶标
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功能
Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic. -
组织特异性
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. -
序列相似性
Belongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain. -
细胞定位
Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5337 Human
- Entrez Gene: 18805 Mouse
- Entrez Gene: 25096 Rat
- Omim: 602382 Human
- SwissProt: Q13393 Human
- SwissProt: Q9Z280 Mouse
- SwissProt: P70496 Rat
- Unigene: 382865 Human
see all -
别名
- Choline phosphatase 1 antibody
- hPLD1 antibody
- Phosphatidylcholine-hydrolyzing phospholipase D1 antibody
see all
图片
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All lanes : Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) (RIPA) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) (RIPA) whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) (RIPA) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 60 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBS
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Anti-Phospholipase D1 antibody [EP1506Y] (ab68150) at 1/500 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 120 kDa
Observed band size: 120 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab68150 被引用在 3 文献中.
- Stricker HM et al. The phospholipase D inhibitor FIPI potently blocks EGF-induced calcium signaling in human breast cancer cells. Cell Commun Signal 19:43 (2021). PubMed: 33832505
- Wang Z et al. Binding of PLD2-Generated Phosphatidic Acid to KIF5B Promotes MT1-MMP Surface Trafficking and Lung Metastasis of Mouse Breast Cancer Cells. Dev Cell 43:186-197.e7 (2017). PubMed: 29033361
- Sulzmaier FJ et al. PEA-15 potentiates H-Ras-mediated epithelial cell transformation through phospholipase D. Oncogene 31:3547-60 (2012). PubMed: 22105357