重组Anti-Phospholipase C beta 3/PLCB3抗体[EPR5951] (ab124735)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5951] to Phospholipase C beta 3/PLCB3
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Phospholipase C beta 3/PLCB3抗体[EPR5951]
参阅全部 Phospholipase C beta 3/PLCB3 一抗 -
描述
兔单克隆抗体[EPR5951] to Phospholipase C beta 3/PLCB3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HCT 116, 293T, and A431, HeLa cell lysates; Human breast and colon tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5951 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124735于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
靶标
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功能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. -
序列相似性
Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5331 Human
- Omim: 600230 Human
- SwissProt: Q01970 Human
- Unigene: 523761 Human
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别名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase beta-3 antibody
see all
图片
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All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PLCB3 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab124735 observed at 150 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab124735 was shown to react with Phospholipase C beta 3/PLCB3 in wild-type HeLa cells in Western blot with loss of signal observed in PLCB3 knockout cell line ab266034 (PLCB3 knockout cell lysate ab258122). Wild-type HeLa and PLCB3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124735 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (ab124735) at 1/1000 dilution
Lane 1 : HCT 116 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 139 kDa -
ab124735 at 1/100 dilution staining Phospholipase C beta 3/PLCB3 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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