Application
Western blot
Sample
Human Cell lysate - whole cell (Astrocytes Cell Culture)
Specification
Astrocytes Cell Culture
Gel Running Conditions
Reduced Denaturing (12% T)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit Ge Healthsciences
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Additional data
Additional Notes
PVDF membrane.
Transfer conditions: 100V 60 minutes
Transfer conditions: 100V 60 minutes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Daniel Martins-de-Souza
Verified customer
提交于 Jan 26 2009