Application
Western blot
Sample
Cow Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (Used any kD precast gel)
Loading amount
25 µg
Specification
Liver
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 30 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/40000
Name
Non-Abcam antibody was used: Anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 23 kDa
Positive control
1 sample (bovine liver tissue lysate) that was not part of the trial
Additional data
Additional Notes
This review is submitted in fulfillment of .
Each lane in the uploaded gel image is bovine liver tissue lysate. Each lane is a separate individual from our trial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 23 2016