Application
Western blot
Sample
Pig Tissue lysate - other (Muscle sarcoplasmic extract)
Gel Running Conditions
Reduced Denaturing (12.5% acrylamide)
Loading amount
62.5 µg
Specification
Muscle sarcoplasmic extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween
Dilution
1/20000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Pierce 31460
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Prime
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Additional data
Additional Notes
Image Key: Lane 1 and 2 - 1:10,000 dilution of primary antibody, Lane 4 and 5 - 1:20,000 dilution of primary antibody, Lane 7 and 8 - Secondary control (no primary antibody)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Shannon Cruzen
Verified customer
提交于 Feb 05 2014