Anti-Peroxiredoxin 1/PAG抗体(ab59538)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 1/PAG
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Peroxiredoxin 1/PAG抗体
参阅全部 Peroxiredoxin 1/PAG 一抗 -
描述
兔多克隆抗体to Peroxiredoxin 1/PAG -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Cow, Cynomolgus monkey, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59538于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/4000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/4000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
序列相似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
翻译后修饰
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 281997 Cow
- Entrez Gene: 5052 Human
- Entrez Gene: 117254 Rat
- Omim: 176763 Human
- SwissProt: Q9JKY1 Chinese hamster
- SwissProt: Q5E947 Cow
- SwissProt: Q06830 Human
- SwissProt: Q63716 Rat
see all -
别名
- Heme binding 23 kDa protein antibody
- MSP23 antibody
- Natural killer cell-enhancing factor A antibody
see all
图片
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ab59538 at 1/100 dilution staining Peroxiredoxin 1/PAG in Non-ciliated bronchiolar cells (Clara cells) of rat airway by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
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ab59528 at 1/100 dilution staining Peroxiredoxin 1/PAG in Rat kidney tubules by Immunofluorescence. Secondary antibody Jackson Cy3 (1/200).
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All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab59538) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab59538 被引用在 16 文献中.
- Peugnet V et al. Mitochondrial-Targeted Therapies Require Mitophagy to Prevent Oxidative Stress Induced by SOD2 Inactivation in Hypertrophied Cardiomyocytes. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453408
- Meyer MT et al. Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors. Int J Mol Sci 22:N/A (2021). PubMed: 34360635
- Wang S et al. Danhong Injection Protects Hemorrhagic Brain by Increasing Peroxiredoxin 1 in Aged Rats. Front Pharmacol 11:346 (2020). PubMed: 32292340
- Dai X et al. Peroxiredoxin 1 protein interacts with influenza virus ribonucleoproteins and is required for efficient virus replication. Vaccine 36:4540-4547 (2018). PubMed: 29921490
- Bhopale KK et al. Proteomic Profiling of Liver and Plasma in Chronic Ethanol Feeding Model of Hepatic Alcohol Dehydrogenase-Deficient Deer Mice. Alcohol Clin Exp Res 41:1675-1685 (2017). PubMed: 28792616