Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain, cerebellum)
Specification
Brain, cerebellum
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton
Blocking step
Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Vector BA 9010
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500
Additional data
Additional Notes
Biotin detected with streptavidin Alexa Fluor 594. The image shows a climbing fiber in the cerebellar molecular layer.
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提交于 Feb 18 2010