Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (HCT116)
Specification
HCT116
Permeabilization
Yes - when Paraformaldehyde
Fixative
Methanol
Other product details
Concentration
1 µg/ml
Incubation time
0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse Alexa Flour 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
I co-stained pericentrin (ab28144) with a tested centrosome antibody (CDK5RAP2, rabbit primary antibody) and found that they obviously co-localized at centrosome in both methenol and para fixed HCT116.
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提交于 Oct 17 2014