Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymphoid tissue)
Specification
Lymphoid tissue
Fixative
Zinc
Antigen retrieval step
None
Permeabilization
No
Blocking step
Rodent block-M (other company) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS + 0.05% Tween20
Secondary antibody
Name
Non-Abcam antibody was used: HRP Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Zinc-based fixative:
0.5% zinc chloride,
0.5% zinc acetate,
0.05% calcium acetate in
0.1M Tris–HCl pH 6.4–6.7
0.5% zinc chloride,
0.5% zinc acetate,
0.05% calcium acetate in
0.1M Tris–HCl pH 6.4–6.7
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 28 2009