Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Other product details
Dilution
1/150
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: CasBlock Solution
Secondary antibody
Name
Non-Abcam antibody was used: Donkey Anti-Mouse IgG (H+L)
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Dilution
1/500
Additional data
Abcam response
Thank you for your valuable feedback. We have not tested this antibody with mouse in our own labs, and it is possible that it does not work well with this species. We offered the customer a discount to test this antibody in mouse samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 11 2011