Anti-PYK2抗体(ab102074)
Key features and details
- Rabbit polyclonal to PYK2
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PYK2抗体
参阅全部 PYK2 一抗 -
描述
兔多克隆抗体to PYK2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Horse, Pig, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human PYK2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab121091) -
阳性对照
- This antibody gave a positive signal in Rat Brain and Rat Hippocampus tissue lysates, as well as in the following whole cell lysates: Jurkat; K562.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption. -
组织特异性
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity. -
细胞定位
Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase. - Information by UniProt
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数据库链接
- Entrez Gene: 2185 Human
- Entrez Gene: 19229 Mouse
- Entrez Gene: 50646 Rat
- Omim: 601212 Human
- SwissProt: Q14289 Human
- SwissProt: Q9QVP9 Mouse
- SwissProt: P70600 Rat
- Unigene: 491322 Human
see all -
别名
- CADTK antibody
- CAK-beta antibody
- CAKB antibody
see all
图片
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All lanes : Anti-PYK2 antibody (ab102074) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Hippocampus Tissue Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Exposure time: 2 minutes -
ICC/IF image of ab102074 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102074 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab102074 尚未被引用在任何文献中。