Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cultured Cells (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,25% Triton in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Donkey serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Cy3
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Dilution
1/500
Additional data
Additional Notes
Very nice, bright staining. However, very diffuse. Suprisingly not in the nucleus even though Pur Alpha is known to be located there.
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提交于 Dec 30 2010