Anti-PUF60/FIR抗体(ab22819)
Key features and details
- Goat polyclonal to PUF60/FIR
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PUF60/FIR抗体
参阅全部 PUF60/FIR 一抗 -
描述
山羊多克隆抗体to PUF60/FIR -
宿主
Goat -
特异性
ab22819 recognises both reported human isoforms of PUF60/FIR
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经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Pig -
免疫原
Synthetic peptide corresponding to Human PUF60/FIR aa 530-542 (C terminal).
Sequence:YDQERFDNSDLSA
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阳性对照
- A431 lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab22819 has bee purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22819于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 58 kDa.
1 hour primary incubation is recommended for this product. |
说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 58 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA. -
组织特异性
Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
序列相似性
Belongs to the RRM half pint family.
Contains 3 RRM (RNA recognition motif) domains. -
结构域
The third RNA recognition motif, called PUMP domain, is atypical and may rather mediate homodimerization and/or protein-protein interactions. -
细胞定位
Nucleus. Colocalizes partially with TROVE2. - Information by UniProt
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数据库链接
- Entrez Gene: 22827 Human
- Entrez Gene: 67959 Mouse
- Entrez Gene: 84401 Rat
- Omim: 604819 Human
- SwissProt: Q9UHX1 Human
- SwissProt: Q3UEB3 Mouse
- SwissProt: Q9WV25 Rat
- Unigene: 521924 Human
see all -
别名
- 60 kDa poly(U)-binding-splicing factor antibody
- FBP-interacting repressor antibody
- FIR antibody
see all
图片
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Anti-PUF60/FIR antibody (ab22819) at 0.1 µg/ml + HEK293 cell lysate (35µg in RIPA buffer)
Predicted band size: 58 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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ICC/IF image of ab22819 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22819, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Anti-PUF60/FIR antibody (ab22819) at 1 µg/ml + A431 lysate (35µg protein in RIPA buffer).
Predicted band size: 58 kDa
Observed band size: 58 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab22819 被引用在 3 文献中.
- Drongitis D et al. Deregulation of microtubule organization and RNA metabolism in Arx models for lissencephaly and developmental epileptic encephalopathy. Hum Mol Genet 31:1884-1908 (2022). PubMed: 35094084
- Sun D et al. PUF60 accelerates the progression of breast cancer through downregulation of PTEN expression. Cancer Manag Res 11:821-830 (2019). PubMed: 30697074
- Corsini L et al. Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60. J Biol Chem 284:630-9 (2009). WB . PubMed: 18974054