Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Sample
Human Tissue sections (Lymph node)
Specification
Lymph node
Permeabilization
No
Fixative
Acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBS with 1% FBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-rabbit IgG (H+L) Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jul 15 2013