Application
Western blot
Sample
Mouse Cell lysate - other (cerebellum, kidney)
Gel Running Conditions
Reduced Denaturing (8% gel)
Loading amount
70 µg
Specification
cerebellum, kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: Mouse Anti-Goat IgG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 101 kDa Non-specific: everywhere kDa
Positive control
my samples
Additional data
Abcam response
This antibody was tested in mouse samples as part of the AbTrial program. We have not tested mouse samples in our lab and would therefore encourage those researchers interested in testing novel species or applications to use our AbTrial program. Please contact our Scientific Support Team for more information.
Also, background and non-specific staining could potentially be reduced by using a secondary antibody that was not raised in a host species that is the same as one's sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mike Liu
Verified customer
提交于 Apr 17 2015