PE Anti-PSMA抗体[GCP-05] (ab77228)
Key features and details
- PE Mouse monoclonal [GCP-05] to PSMA
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
PE Anti-PSMA抗体[GCP-05]
参阅全部 PSMA 一抗 -
描述
PE小鼠单克隆抗体[GCP-05] to PSMA -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab77228 recognizes extracellular domain of PSMA, expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 44-750 of Human PSMA
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常规说明
The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Size exclusion -
克隆
单克隆 -
克隆编号
GCP-05 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77228于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 5 µg/ml.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use a concentration of 5 µg/ml. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. -
组织特异性
Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. -
序列相似性
Belongs to the peptidase M28 family. M28B subfamily. -
结构域
The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. -
翻译后修饰
The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked. -
细胞定位
Cytoplasm and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2346 Human
- Omim: 600934 Human
- SwissProt: Q04609 Human
- Unigene: 654487 Human
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别名
- Cell growth inhibiting protein 27 antibody
- Cell growth-inhibiting gene 27 protein antibody
- FGCP antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab77228 被引用在 8 文献中.
- Glud EN et al. Identification of a high-risk immunogenic prostate cancer patient subset as candidates for T-cell engager immunotherapy and the introduction of a novel albumin-fused anti-CD3 × anti-PSMA bispecific design. Br J Cancer 127:2186-2197 (2022). PubMed: 36243890
- Papasotiriou I et al. Study and detection of potential markers for predicting metastasis into lymph nodes in prostate cancer. Biomark Med 14:1317-1327 (2020). PubMed: 32799659
- Yunger S et al. Tumor-infiltrating lymphocytes from human prostate tumors reveal anti-tumor reactivity and potential for adoptive cell therapy. Oncoimmunology 8:e1672494 (2019). PubMed: 31741775
- Minn I et al. Imaging CAR T cell therapy with PSMA-targeted positron emission tomography. Sci Adv 5:eaaw5096 (2019). PubMed: 31281894
- Sácha P et al. Expression of glutamate carboxypeptidase II in human brain. Neuroscience 144:1361-72 (2007). PubMed: 17150306
- Barinka C et al. Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem 271:2782-90 (2004). PubMed: 15206943
- Barinka C et al. Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci 13:1627-35 (2004). PubMed: 15152093
- Barinka C et al. Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem 80:477-87 (2002). PubMed: 11905994