Anti-PSD95抗体[6G6-1C9] (ab2723)

概述

  • 产品名称
    Anti-PSD95抗体[6G6-1C9]
    参阅全部 PSD95 一抗
  • 描述
    小鼠单克隆抗体[6G6-1C9] to PSD95
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-FoFr, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Zebrafish
    预测可用于: Cow
  • 免疫原

    Recombinant full length protein corresponding to PSD95.

  • 阳性对照
    • In Western Blot, this antibody gave a positive signal in rat and mouse forebrain and hippocampus tissue lysates.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Protocols tab contains a Mouse on Mouse staining protocol with recommendations when using a mouse monoclonal antibody to stain mouse tissues and tips for reducing background.

性能

应用

Our Abpromise guarantee covers the use of ab2723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr 1/3000. PubMed: 22539981
IP Use at an assay dependent concentration.

靶标

  • 功能
    Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 组织特异性
    Brain.
  • 序列相似性
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域
    The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻译后修饰
    Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 细胞定位
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

图片

  • Lanes 1 - 6 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution
    Lanes 7 - 12 : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 1/1200 dilution (18 hours at 4°C)

    Lane 1 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 2 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 3 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 4 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 5 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 6 : Mouse Hippocampus with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 7 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 8 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 9 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 10 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 11 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C
    Lane 12 : Mouse Liver with Li-Cor Block Buffer, 1 hour at 21°C

    Lysates/proteins at 30 µg per lane.

    Secondary
    Li-Cor IRDye® Donkey anti-mouse 680LT at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    Image courtesy of an AbReview submitted by Grant Corbett

    See Abreview

  • ab2723 staining cultured rat primary hippocampal neurons by ICC/IF. The cultured neurons were fixed with 4% formaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS- 0.3% TritonX for 30 minutes at 24°C. The cultured neurons were then stained with ab2723 at 1/1000 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 568 donkey anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. PSD95 (which stains the axons and dendrytic spines) can be observed in red (Alexa fluro 568 secondary). Synaptophysin is observable in green (alexa 488 secondary) and colocalizes with PSD95 in dendritic spines. Nuclei are stained in blue with 1.43µM Hoechst.

    See Abreview

  • All lanes : Anti-PSD95 antibody [6G6-1C9] (ab2723) at 5 µg/ml

    Lane 1 : Hippocampus (Rat) Tissue Lysate
    Lane 2 : Forebrain (Rat) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Forebrain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,110 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ab2723 staining PSD95 in Zebrafish retina (outer plexiform layer) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour at 23°C. Sodium citrate antigen retrieval was used. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab2723 at 1/200 staining rat primary hippocampal neurons by ICC/IF. The cells were paraformaldehyde fixed and then permeabilized with 0.1% Triton X-100 before being stained with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat polyclonal antibody was used as the secondary.

    The antibody shows numerous puncta along the shafts.

    See Abreview



  • Predicted band size : 80 kDa

    This image is courtesy of an anonymous Abreview

    All lanes : PSD95 antibody (ab2723) at 1/2000 dilution (in blocking buffer for 16 hours at 4°C) + whole tissue lysate of Mouse hippocampus at 10µg

    Secondary
    An HRP-conjugated Horse anti-mouse IgG polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions

    Exposure time : 30 seconds

    Blocking Step : 5% Milk for 1 hour at 25°C

    See Abreview

  • IHC-FoFr staining of PSD95 on rat hippocampus section using ab2723 (1:3000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

文献

This product has been referenced in:
  • Cacciottolo M  et al. Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models. Transl Psychiatry 7:e1022 (2017). Read more (PubMed: 28140404) »
  • Bae JS  et al. Lycopersicon esculentum Extract Enhances Cognitive Function and Hippocampal Neurogenesis in Aged Mice. Nutrients 8:N/A (2016). Read more (PubMed: 27792185) »

See all 62 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (retina, outer plexiform layer)
Specification
retina, outer plexiform layer
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

提交于 Oct 02 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% in TGS)
Sample
Mouse Tissue lysate - whole (Hippocampus and Liver)
Specification
Hippocampus and Liver
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Grant Corbett

Verified customer

提交于 Jul 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Mouse Tissue lysate - whole (mouse brain tissue)
Specification
mouse brain tissue
Total protein in input
200 µg
Username

Abcam user community

Verified customer

提交于 Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse hippocampus and cerebellum)
Specification
mouse hippocampus and cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jul 04 2012

We do not know how PSD95 expression would be affected if toxicity is increased in cells. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their s...

Read More

Thank you for contacting Abcam.

In mouse neurons an alternative to the smaller housekeeping proteins such as beta-actin, or GAPDH commonly used as loading control would be to use synaptic markers such as PSD95 or Piccolo.

In weste...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Hippocampal neurons)
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Specification
Rat Hippocampal neurons
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Apr 12 2012

Thank you for contacting us.

To our knowledge, the epitope this clone recognizes has not been mapped. The immunogen was full-length rat PSD95.

If you do decide to try this antibody, we would of course be very interested in the res...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Brain Homogenate)
Loading amount
50 µg
Specification
Brain Homogenate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 14 2012

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册