Anti-PRUNE抗体(ab88613)
Key features and details
- Mouse polyclonal to PRUNE
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PRUNE抗体
参阅全部 PRUNE 一抗 -
描述
小鼠多克隆抗体to PRUNE -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein within Human PRUNE. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: Raw 264.7 cell lysate, NIH/3T3 cell lysate, and PRUNE transfected 293T cell lysate ICC/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88613于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. -
组织特异性
Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. -
序列相似性
Belongs to the PPase class C family. Prune subfamily. -
细胞定位
Cytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed. - Information by UniProt
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数据库链接
- Entrez Gene: 58497 Human
- Entrez Gene: 229589 Mouse
- SwissProt: Q86TP1 Human
- SwissProt: Q8BIW1 Mouse
- Unigene: 728223 Human
- Unigene: 14155 Mouse
- Unigene: 279287 Mouse
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别名
- DRES-17 antibody
- DRES17 antibody
- Drosophila-related expressed sequence 17 antibody
see all
图片
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Anti-PRUNE antibody (ab88613) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-PRUNE antibody (ab88613) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PRUNE antibody (ab88613) at 1 µg/ml
Lane 1 : PRUNE transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab88613, at 10 µg/ml, staining PRUNE in HeLa cell by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab88613 被引用在 2 文献中.
- Santoro D et al. Evaluation of the expression and distribution of Prune-1 in the skin of healthy dogs. Vet Dermatol 30:446-447 (2019). PubMed: 31286578
- Zollo M et al. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain 140:940-952 (2017). IF . PubMed: 28334956