Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepG2)
Loading amount
20 µg
Specification
293T, HepG2
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Other product details
Dilution
1/10000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP-conjugated anti-rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 73 kDa Non-specific: 55, 40 kDa
Positive control
-
Negative control
-
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dongil Kim
Verified customer
提交于 Feb 01 2013