重组HRP Anti-PPP1CA + PPP1CB抗体[EP1511Y] (ab211372)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1511Y] to PPP1CA + PPP1CB
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-PPP1CA + PPP1CB抗体[EP1511Y]
参阅全部 PPP1CA + PPP1CB 一抗 -
描述
HRP兔单克隆抗体[EP1511Y] to PPP1CA + PPP1CB -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa whole cell and Mouse Brain and Rat Brain tissue lysates.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1511Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab211372于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). |
靶标
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细胞定位
Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. -
数据库链接
- Entrez Gene: 5499 Human
- Entrez Gene: 5500 Human
- Entrez Gene: 19045 Mouse
- Entrez Gene: 19046 Mouse
- Entrez Gene: 24668 Rat
- Entrez Gene: 25594 Rat
- Omim: 176875 Human
- Omim: 600590 Human
see all -
别名
- PP-1A antibody
- PP-1B antibody
- PP1A antibody
see all
图片
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All lanes : HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (ab211372) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 :HeLa whole cell lysate (ab150035)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab211372 被引用在 1 文献中.
- Hu Z et al. Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids. PLoS Pathog 16:e1008669 (2020). PubMed: 32702076