Anti-PPM1A抗体[p6c7] (ab14824)
Key features and details
- Mouse monoclonal [p6c7] to PPM1A
- Suitable for: ELISA, IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-PPM1A抗体[p6c7]
参阅全部 PPM1A 一抗 -
描述
小鼠单克隆抗体[p6c7] to PPM1A -
宿主
Mouse -
经测试应用
适用于: ELISA, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein (Human).
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阳性对照
- WB: HeLa, HAP1, Jurkat, K562, MCF7, A549 and Raji cell lysates; Mouse kidney, brain and liver lysates; Mouse liver cytosol extract. ICC: HeLa cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 28/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
p6c7 -
骨髓瘤
Sp2/0 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14824于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
1/100.
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WB | (3) |
1/250 - 1/1000. Predicted molecular weight: 42 kDa.
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ICC/IF |
1/100.
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说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. |
WB
1/250 - 1/1000. Predicted molecular weight: 42 kDa. |
ICC/IF
1/100. |
靶标
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功能
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. -
序列相似性
Belongs to the PP2C family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5494 Human
- Entrez Gene: 19042 Mouse
- Omim: 606108 Human
- SwissProt: P35813 Human
- SwissProt: P49443 Mouse
- Unigene: 130036 Human
- Unigene: 261045 Mouse
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别名
- Protein phosphatase 2C isoform alpha antibody
- EC 3.1.3.16 antibody
- FLJ42306 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of PP2C alpha/PPM1A in HeLa cells. The cell was stained with ab14824 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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All lanes : Anti-PPM1A antibody [p6c7] (ab14824) at 1/500 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : PPM1A knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HAP1 whole cell lyate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaLanes 1-4: Merged signal (red and green). Green - ab14824 observed at 42 kDa. Red - loading control ab181602 observed at 36 kDa.
ab14824 Anti-PPM1A antibody [p6c7] was shown to specifically react with PPM1A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265348 (knockout cell lysate ab259055) was used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab14824 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PPM1A knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab14824 observed at 42 kDa. Red - loading control, ab18251, observed at 52 kDa.
ab14824 was shown to specifically react with PPM1A when PPM1A knockout samples were used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab14824 diluted to 1/250 and ab18251 (loading control to alpha Tubulin) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.This image was generated using the ascites version of the product.
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All lanes : Anti-PPM1A antibody [p6c7] (ab14824) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : Raji cell lysate
Lane 7 : Mouse kidney tissue lysate
Lane 8 : Mouse brain tissue lysate
Lane 9 : Mouse liver tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 42 kDa -
Western blot analysis of mouse liver cytosol extract using ab14824 at a dilution of 1/250. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Western blot analysis of mouse liver cytosol extract using ab14824 at a dilution of 1/250. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.
This image was generated using the ascites version of the product.
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Anti-PPM1A antibody [p6c7] (ab14824) at 1/1000 dilution + HeLa whole cell lysate
Secondary
HRP conjugated anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis image is courtesy of an Abreview submitted by Xia Lin on 2 March 2006.
This image was generated using the ascites version of the product.
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Ab14824 staining human colon. Staining is localised to cytoplasm.
Left panel: with primary antibody at 4ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.This image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab14824 被引用在 17 文献中.
- Feng D et al. Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage. Sci Adv 8:eabq2423 (2022). PubMed: 36179025
- Xia YC et al. lncRNA TSPEAR-AS2, a Novel Prognostic Biomarker, Promotes Oral Squamous Cell Carcinoma Progression by Upregulating PPM1A via Sponging miR-487a-3p. Dis Markers 2021:2217663 (2021). PubMed: 34336002
- Yang J et al. Knockdown of Mg2+/Mn2+ dependent protein phosphatase 1A promotes apoptosis in BV2 cells infected with Brucella suis strain 2 vaccine. Exp Ther Med 20:926-932 (2020). PubMed: 32742335
- Wang Z et al. MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation. Allergy Asthma Immunol Res 12:859-876 (2020). PubMed: 32638565
- Wang K et al. TGF-ß1/p65/MAT2A pathway regulates liver fibrogenesis via intracellular SAM. EBioMedicine 42:458-469 (2019). PubMed: 30926424