Application
Western blot
Sample
Mouse Tissue lysate - whole (brain cortex)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
20 µg
Specification
brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 150, 60, 30 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 29 2015