Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080 Cell line)
Specification
HT1080 Cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Other product details
Concentration
10 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 488 Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in IF. This antibody has not been tested with the HT1080 cell line previously and your data suggest it may not be suitable for these cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 15 2010