Anti-PP1C gamma抗体(ab16387)
Key features and details
- Sheep polyclonal to PP1C gamma
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PP1C gamma抗体
参阅全部 PP1C gamma 一抗 -
描述
羊多克隆抗体to PP1C gamma -
宿主
Sheep -
特异性
No cross reactivity with other recombinant pp1 isoforms. -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Xenopus laevis -
免疫原
Synthetic peptide:
TPPRGMITKQAKK
conjugated to KLH, corresponding to amino acids 311-323 of Human PPP1G1. -
阳性对照
- Recombinant Human PP1C gamma protein (ab114828) can be used as a positive control in WB.
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常规说明
A protein phosphatase is a phosphatase enzyme that removes a phosphate group from the phosphorylated amino acid residue of its substrate protein. Protein phosphorylation is one of the most common forms of reversible protein posttranslational modification (PTM), with up to 30% of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyse the transfer of a γ-phosphate from ATP to specific amino acids on proteins. Several hundred PKs exist in mammals and are classified into distinct super-families. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 79.3, 16.9 and 3.8% respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. The largest class of PPs is the phosphoprotein phosphatase (PPP) family comprising PP1, PP2A, PP2B, PP4, PP5, PP6 and PP7, and the protein phosphatase Mg2+ or Mn2+-dependent (PPM) family, composed primarily of PP2C.
Source: The immunogen used to generate the purified antibody was a peptide conjugated to KLH corresponding to the sequence NH2-Thr-Pro-Pro-Arg-Gly-Met-Ile-Thr-Lys-Gln-Ala-Lys-Lys-COOH. This peptide antibody corresponds to C -terminal peptide of PP1 gamma 1 catalytic subunit having a MW of 37kD. The sequence used is amino acid 311-323.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16387于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
|
说明 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. -
序列相似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
细胞定位
Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus speckle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. Colocalizes with SPZ1 in the nucleus (By similarity). Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles. Shows a dynamic targeting to specific sites throughout the cell cycle. Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis. Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase. Also accumulates at the cleavage furrow and midbody by telophase. - Information by UniProt
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数据库链接
- Entrez Gene: 282318 Cow
- Entrez Gene: 5501 Human
- Entrez Gene: 19047 Mouse
- Entrez Gene: 24669 Rat
- Omim: 176914 Human
- SwissProt: P61287 Cow
- SwissProt: P36873 Human
- SwissProt: P63087 Mouse
see all -
别名
- PP 1G antibody
- PP-1G antibody
- PP1C antibody
see all
数据表及文件
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Datasheet download
文献 (5)
ab16387 被引用在 5 文献中.
- Verdugo-Sivianes EM et al. Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes the tumorigenic and stemness properties of cells. Theranostics 11:3452-3471 (2021). PubMed: 33537097
- Seiki T et al. HPV-16 impairs the subcellular distribution and levels of expression of protein phosphatase 1? in cervical malignancy. BMC Cancer 15:230 (2015). PubMed: 25886518
- Kernohan KD et al. Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability. Hum Mol Genet 24:6293-300 (2015). WB, IP ; Human . PubMed: 26307080
- McCoy F et al. Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts. J Biol Chem 288:8838-48 (2013). Xenopus laevis . PubMed: 23400775
- Aoyama H et al. Isoform-specific roles of protein phosphatase 1 catalytic subunits in sarcoplasmic reticulum-mediated Ca(2+) cycling. Cardiovasc Res 89:79-88 (2011). PubMed: 20675715