Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 cell lines)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Treatment
Expression seen with MLH1 expression
Specification
HCT116 cell lines
Blocking step
odyssey PBS as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: 37°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Detection
Detection method
Odyssey system
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~100 kDa
Additional data
Additional Notes
lane 1 is ctr vector and lane 2 is mlh1 expression in hct-116, leading to pms2 expression
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 14 2015