Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Nasal polyp, tonsil)
Specification
Nasal polyp, tonsil
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 21°C
Dilution
1/20
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Biotinylated rabbit anti mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
The tissue was fixed in acetone and inbitors and embedded into GMA resin as per the Southampton HRU protocol.
The primary antibody is incubated overnight at room temperature.
The antibody was tested at a range of dilutions from 1:5 to 1:400.
At 1:20, there was some clear staining. Unfortunately a matched isotype control is strongly positive for this concentration.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 12 2011