Anti-PKC mu/PKD (phospho S916)抗体[EP1494Y] (ab81218)
Key features and details
- Rabbit monoclonal [EP1494Y] to PKC mu/PKD (phospho S916)
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-PKC mu/PKD (phospho S916)抗体[EP1494Y]
参阅全部 PKC mu/PKD 一抗 -
描述
兔单克隆抗体[EP1494Y] to PKC mu/PKD (phospho S916) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: lysate from HeLa cells treated with TPA. IHC: Human placenta tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1494Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81218于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/50000 - 1/100000. Predicted molecular weight: 102 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/60.
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说明 |
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WB
1/50000 - 1/100000. Predicted molecular weight: 102 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/60. |
靶标
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功能
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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数据库链接
- Entrez Gene: 5587 Human
- Omim: 605435 Human
- SwissProt: Q15139 Human
- Unigene: 508999 Human
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别名
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
图片
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Serum starved HeLa cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of phorbol 12-myristate 13-acetate (PMA) (ab120297) in DMSO. Increased expression of PKC mu/PKD (phospho S916) (ab81218) correlates with an increase in phorbol 12-myristate 13-acetate (PMA) concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab81218 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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All lanes : Anti-PKC mu/PKD (phospho S916) antibody [EP1494Y] (ab81218) at 1/100000 dilution
Lane 1 : lysate from untreated HeLa cells
Lane 2 : lyaste from HeLa cells treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 102 kDa -
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 ab81218.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab81218 被引用在 2 文献中.
- Li Z et al. E-Cadherin Facilitates Protein Kinase D1 Activation and Subcellular Localization. J Cell Physiol 231:2741-8 (2016). PubMed: 26991955
- Harikumar KB et al. A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo. Mol Cancer Ther 9:1136-46 (2010). PubMed: 20442301