Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody did not produce the expected cytoplasmic staining pattern in these cells under these conditions. MeOH fixed samples were also evaluated and did not appear to identify any epitope under these conditions.
Abcam response
Thank you for your valuable feedback. Your data suggests that ab108961 may not be suitable to detect endogenous PKC gamma by ICC/IF in HeLa cells under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 28 2014