Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Guinea pig Cell lysate - other (cardiac myocyte)
Specification
cardiac myocyte
Treatment
100nM drug1 for 30min, 1uM drug1 for 30min, 10uM drug1 for 30min, 100uM drug1 for 15min, 100uM drug1 for 30min, 100uM drug1 for 60min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 5°C · Diluent: bsa
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: peroxidase -conjugated affinipure goat anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ecl
Bands
Specific: 84 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
100nM drug1 for 30min, 1uM drug1 for 30min, 10uM drug1 for 30min, 100uM drug1 for 15min, 100uM drug1 for 30min, 100uM drug1 for 60min
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提交于 Jul 07 2014