Application
Western blot
Sample
Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Loading amount
40 µg
Specification
C57BL/6 Mouse splenocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA 2% Milk in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG (H&L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
SuperSignal West Femto
Exposure
10 second(s)
Bands
Specific: 77 kDa Non-specific: 78 kDa
Additional data
Additional Notes
Antibody recognized two proteins which appear to be a doublet at 77 and 78kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Tomar Ghansah
Verified customer
提交于 Oct 12 2012