Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Antigen retrieval step
None
Permeabilization
No
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Donkey Serum, BSA, Triton X-100, PBS
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
Eyeballs were fixed in 4% Paraformaldehyde for 2h.
Immunoreactivity in the mole-rat retina was observed exclusively in bipolar cells with this highly specific AB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 21 2016