Anti-PIWIL4/PIWI抗体(ab87939)
Key features and details
- Rabbit polyclonal to PIWIL4/PIWI
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIWIL4/PIWI抗体
参阅全部 PIWIL4/PIWI 一抗 -
描述
兔多克隆抗体to PIWIL4/PIWI -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PIWIL4/PIWI.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab87939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/1000. Predicted molecular weight: 97 kDa.
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IHC-P |
1/50 - 1/200.
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说明 |
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WB
1/200 - 1/1000. Predicted molecular weight: 97 kDa. |
IHC-P
1/50 - 1/200. |
靶标
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功能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. -
组织特异性
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. -
序列相似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
翻译后修饰
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
细胞定位
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization. - Information by UniProt
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数据库链接
- Entrez Gene: 143689 Human
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- Unigene: 660188 Human
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别名
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
图片
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All lanes : Anti-PIWIL4/PIWI antibody (ab87939) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal testis cell lysate
Predicted band size: 97 kDa -
ab87939 at 1/100 dilution, staining PIWIL4/PIWI in formalin-fixed paraffin-embedded testis tissue showing nuclear staining.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab87939 被引用在 3 文献中.
- Subhramanyam CS et al. Role of PIWI-like 4 in modulating neuronal differentiation from human embryonal carcinoma cells. RNA Biol 17:1613-1624 (2020). PubMed: 32372724
- Rocha-da-Silva L et al. Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics. PLoS One 14:e0217941 (2019). PubMed: 31181099
- Zhong F et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res 43:10474-91 (2015). WB . PubMed: 26405199