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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MILI/ HILI.
(Peptide available as ab27821.)
Our Abpromise guarantee covers the use of ab36764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20534472|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).Can be blocked with Mouse PIWIL2 peptide (ab27821).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20460113
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20460113|
|IP||Use at an assay dependent concentration. PubMed: 20460113|
|Electron Microscopy||Use at an assay dependent concentration. PubMed: 20011505|
Testes section from adult mice were immunostained with anti-PIWIL2 (MILI) antibody ab36764. Nuclei were stained with DAPI. Testes were fixed in 4% PFA at 4°C for 3 hours and overnight respectively, dehydrated in 30% sucrose overnight, frozen in dry ice/95% ethanol, and sectioned at –20°C.
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