Anti-PIM1 (phospho Y309)抗体(ab74047)
Key features and details
- Rabbit polyclonal to PIM1 (phospho Y309)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIM1 (phospho Y309)抗体
参阅全部 PIM1 一抗 -
描述
兔多克隆抗体to PIM1 (phospho Y309) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PIM1 (phospho Y309).
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阳性对照
- HUVEC cell extracts treated with PMA (125ng/ml, 30mins).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Frozen Stock (-20C). Shelf life 12 months. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab74047 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74047于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
靶标
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功能
May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. -
组织特异性
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on both serine/threonine and tyrosine residues. -
细胞定位
Cytoplasm. Nucleus and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5292 Human
- Entrez Gene: 18712 Mouse
- Entrez Gene: 24649 Rat
- Omim: 164960 Human
- SwissProt: P11309 Human
- SwissProt: P06803 Mouse
- SwissProt: P26794 Rat
- Unigene: 81170 Human
see all -
别名
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
see all
图片
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All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution
Lane 1 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins)
Lane 2 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab74047 尚未被引用在任何文献中。