Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (8.5%)
Sample
Mouse Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: HRP-coupled goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 52 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
The antibody detects endogenouse Pick1 in homogenate of the brain as well as in the LP2 fraction (8-10x enrichment of synaptic vesicles) but it is not enriched.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 01 2014