Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cultured Cells (CHO Cells stably transfected with human Pick1)
Specification
CHO Cells stably transfected with human Pick1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: PBS, 0.5% Triton, 10% FCS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 488 anit rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
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Abcam user community
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提交于 May 03 2011