Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Mouse Tissue lysate - whole (Brain tissue)
Specification
Brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk+ PBS+0.1% Tween 20
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 85 kDa Non-specific: 55 kDa
Exposure
10 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 20 2014