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Our Abpromise guarantee covers the use of ab13903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab13903 at 1/100 staining human bone Marrow derived CD34+ cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized and blocked with BSA before incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG was used as the secondary. The cells were counterstained with DAPI.
The image shows staining mainly in the cytoplasmic region.
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