Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Rhodamine
Host species: Donkey
Clonality: Polyclonal
Conjugation: Rhodamine
Additional data
Additional Notes
Permeabilizing tissues with 1xTBS/0.1% Triton X-100 at for 2 hrs at 4 degrees.
Blocking: 5% horse serum/1%BSA in TBS at room temp for 1 hr.
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback about the use of ab21685 in IHC-FrFl, an application we have not tested before.We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Trinh Nguyen
Verified customer
提交于 Sep 07 2017