Application
Western blot
Sample
Human Cell lysate - whole cell (fibroblasts)
Loading amount
30 µg
Specification
fibroblasts
Treatment
see below (Additional notes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
chemiluminescence
Exposure
45 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Positive control
pgp9.5 protein
Additional data
Additional Notes
bands:
fibroblasts:
1 = untreated
2 = treated with TGF-ß
3 = treated with TGF-ß and anti-TGF-ß
4 = pgp9.5 protein (positive control)
fibroblasts:
1 = untreated
2 = treated with TGF-ß
3 = treated with TGF-ß and anti-TGF-ß
4 = pgp9.5 protein (positive control)
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提交于 Jul 11 2012