Application
Western blot
Sample
Mouse Tissue lysate - whole (adult brain)
Loading amount
100 µg
Specification
adult brain
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk powder in TBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit POD AffiniPure
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL (Dura)
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 26 kDa
Positive control
As a positive control cell lysates were used that have transiently been transfected with UCH-L1 (Flag-tagged) cDNA. With an anti-Flag antibody this sample has been detected as positive in an earlier experiment.
Additional data
Additional Notes
samples applied on the gel:
1: adult mouse brain homogenate (CD-1 strain)
2: non transfected control cell lysates (low endogenous expression of UCH-L1)
3: cell lysates transfected with UCH-L1-Flag cDNA (positive control)
1: adult mouse brain homogenate (CD-1 strain)
2: non transfected control cell lysates (low endogenous expression of UCH-L1)
3: cell lysates transfected with UCH-L1-Flag cDNA (positive control)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Simone Diestel
Verified customer
提交于 Jan 04 2012