Anti-PGAM2抗体(ab97800)
Key features and details
- Rabbit polyclonal to PGAM2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-PGAM2抗体
参阅全部 PGAM2 一抗 -
描述
兔多克隆抗体to PGAM2 -
宿主
Rabbit -
特异性
The immunogen sequence shows 81% homology with PGAM1, so cross-reactivity with PGAM1 cannot be excluded. However, cross-reactivity has not been tested. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-203 of Human PGAM2 (NP_000281)
-
阳性对照
- H1299 or HeLa lysates A549 cells (IF)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97800于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
-
功能
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
组织特异性
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
疾病相关
Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10) [MIM:261670]. A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. -
序列相似性
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
-
数据库链接
- Entrez Gene: 515067 Cow
- Entrez Gene: 5224 Human
- Entrez Gene: 56012 Mouse
- Entrez Gene: 100188980 Pig
- Entrez Gene: 24959 Rat
- Omim: 612931 Human
- SwissProt: Q32KV0 Cow
- SwissProt: P15259 Human
see all -
别名
- BPG dependent PGAM 2 antibody
- BPG-dependent PGAM 2 antibody
- GSD10 antibody
see all
图片
-
All lanes : Anti-PGAM2 antibody (ab97800) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97800 at 1/200 dilution. Lower image was also merged with DNA probe.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab97800 被引用在 3 文献中.
- Rudolph F et al. Deconstructing sarcomeric structure-function relations in titin-BioID knock-in mice. Nat Commun 11:3133 (2020). PubMed: 32561764
- De Ridder W et al. Multisystem proteinopathy due to a homozygous p.Arg159His VCP mutation: A tale of the unexpected. Neurology 94:e785-e796 (2020). PubMed: 31848255
- Xu Y et al. Oxidative stress activates SIRT2 to deacetylate and stimulate phosphoglycerate mutase. Cancer Res 74:3630-42 (2014). PubMed: 24786789