Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart, skeletal muscle, liver)
Loading amount
60 µg
Specification
Heart, skeletal muscle, liver
Gel Running Conditions
Non-reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1-5 % of BSA
Secondary antibody
Name
Non-Abcam antibody was used: Vector
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
We had to test the antibody-PERK (ab79483) in mouse samples ( heart, skeletal muscle and liver), but the antibody is not working with those samples.
Abcam response
Thank you for your valuable feedback. I am sorry that this antibody did not work in mouse. This antibody has not yet been tested in mouse and may not be suitable.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Mtuhtu S.Gounder
Verified customer
提交于 Dec 21 2012