Anti-PDLIM7抗体(ab86065)
Key features and details
- Rabbit polyclonal to PDLIM7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDLIM7抗体
参阅全部 PDLIM7 一抗 -
描述
兔多克隆抗体to PDLIM7 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Bat -
免疫原
Synthetic peptide corresponding to a region between amino acids 125 and 175 of Human PDLIM7 (NP_005442.2)
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阳性对照
- Hela cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86065于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway. -
组织特异性
Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver. -
序列相似性
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain. -
结构域
The LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
Anchored to cell periphery via its N-terminal PDZ domain. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments. - Information by UniProt
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数据库链接
- Entrez Gene: 462308 Chimpanzee
- Entrez Gene: 533851 Cow
- Entrez Gene: 611246 Dog
- Entrez Gene: 101127125 Gorilla
- Entrez Gene: 100058356 Horse
- Entrez Gene: 9260 Human
- Entrez Gene: 706581 Rhesus monkey
- Omim: 605903 Human
see all -
别名
- 1110003B01Rik antibody
- Enigma antibody
- LIM domain protein antibody
see all
图片
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All lanes : Anti-PDLIM7 antibody (ab86065) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 30 seconds -
Detection of PDLIM7 by Western blot of Immunprecipitate.
ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab86065 被引用在 1 文献中.
- Ajeian JN et al. Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl 10:51-7 (2016). PubMed: 26147903